›› 2009, Vol. 40 ›› Issue (6): 857-861.doi: 10.3969/j.issn.0529-1356.2009.06.002

• 论著 • 上一篇    下一篇

磷酸化细胞外信号调节激酶在海马提取液诱导神经干细胞向神经元分化过程中的表达

朱蕙霞1,3 ;金国华2,3,4*; 田美玲2,3,4; 秦建兵2,3,4; 谭雪锋2,3,4; 金淑仪1   

  1. 1.南通大学医学院 生物化学与分子生物学教研室;2.人体解剖学教研室;3.神经生物学研究所;4.江苏省神经再生重点实验室,南通 226001
  • 收稿日期:2009-06-10 修回日期:2009-07-23 出版日期:2009-12-06
  • 通讯作者: 金国华

Expression of phosphoryl extracellular signalregulated kinases in the differentiation of NSCs into neurons induced by the extracts of the fimbria-transected hippocampi in rat

  1. 1. Department of Biochemistry and Molecule Biology;2. Department of Anatomy; 3. Institute of Neurobiology;4. Nantong University, the Jiangsu Key Laboratory of Neuroregeneration, Nantong 226001, China
  • Received:2009-06-10 Revised:2009-07-23 Online:2009-12-06
  • Contact: JIN Guo-hua

关键词: 切割穹隆海马伞, 海马, 神经干细胞, 分化, 微管相关蛋白2, 磷酸化细胞外信号调节激酶, 免疫荧光双标, 大鼠

Abstract: Objective To investigate the effect of extracellular signal-regulated kinases(ERK) signal transduction in the process of NSCs differentiating into neurons in the fimbria-transected hippocampi’s extracts. Methods Twelve Sprague-Dawley rats’right fimbrias were transected. The extracts were gained from the fimbria-transected hippocampi at the 14th day normal rat, and the extracts supernatant fluid was collected after centrifugal process, then the protein concentration in the extracts was determined. In the serum-free medium,NSCs from the fetal hippocampus were planted on 24 well culture plate, then were divided into three group and eight wells for each group as follows: the transected group contained the extracts of the fimbria-transected hippocampi; the normal group contained the extracts of the normal hippocampi; the pure control group have no extracts. After cultured for 14 days,the cells were detected by using MAP-2 and p-ERK immunofluorescence. Result The number, area, perimeter of MAP-2 positive neurons were all declined in transected group, the normal group and the control group orderly. Statistic results showed significant difference between every two groups. The number of MAP-2/p-ERK double-positive neurons were decreased in transected group, the normal group and the control group orderly, but the percentage of double-labeled neurons in total MAP-2 positive neurons were increased in turn. In these two aspect, there were also significant difference between every two group. And most of the MAP-2/p-ERK double-positive neurons were immature. Conclusion The extracts of the fimbria-transected hippocampi had obvious effects on promoting NSCs differentiating into neurons and speeding up the maturation of neurons than those of the normal hippocampi. The morphological results showed that ERK signal transduction might be related to the differentiation of NSCs i

Key words: Fimbria-fornix transected, Hippocampus, Neural stem cells, Differentiation, Microtubul-associated protein-2, Phosphoryl extracellular signal-regulated kinases, Immunofluorescence double-labelling, Rat

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